Construct: ORF ccsbBroad304_99998

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00005.2_s304c1, BRDN0000412235
Derived from:
BRDN0000464774
DNA Barcode:
None
Vector:
pLX_TRC304
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
eGFP (-44)

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Note
1 CONTROL -44 eGFP eGFP eGFP.1 100% 100% N/A
2 CONTROL -10 GFP clonetechGfp clonetechGfp.1 99.7% 99.5% 93C>T;491T>C
3 CONTROL -40 BFP BFP BFP.1 99.1% 98.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
786
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
121agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg
181ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
241ggcccaccct cgtgaccacc ctgaccTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
301ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
361CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
421ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
481TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC
541AGAAGAACGG CATCAAGGCG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC
601AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG
661ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC
721ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT
781ACAAGTCCAA GGGTGGGCGC GCCGACtttc ttgtacaaag tggttggtaa gcctatccct
841aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
901aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)