Construct: ccsbBroad304_99987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00001.3_s304c1, BRDN0000412224
DNA Barcode:
None
Vector:
pLX_TRC304
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
Hahn Lab BFP

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Note
1 CONTROL -40 BFP BFP BFP.1 99.3% 25% 99delG;116delC;718_720delTTG
2 CONTROL -10 GFP clonetechGfp clonetechGfp.1 98.8% 24.1% (many diffs)
3 CONTROL -44 eGFP eGFP eGFP.1 98.4% 24.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
786
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
121agctggacgg cgacgtaaaC GGCCACAAGT TCAGCGTGTC CGGCGAGGCG AGGGCGATGC
181CACTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
241CCCACCCTCG TGACCACCCT GAGCCACGGC GTCCAGTGCT TCAGCCGCTA CCCCGACCAC
301ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
361ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
421ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
481GGGCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
541AAGAACGGCA TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
601CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
661AGCCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
721ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
781AAGTTGCCAA CTTTCTTGTA Caaagtggtt ggtaagccta tccctaaccc tctcctcggt
841ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac
901ctctggatta

Download FASTA (ORF) (Full)