Construct: ORF ccsbBroad304_99985

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00001.1_s304c1, pLX304-BFP, BRDN0000412222
Derived from:
BRDN0000464762
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BFP (BFP)

Vector Information:

Vector Backbone:
pLX_TRC304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_99985
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 CONTROL BFP BFP Hahn Lab BFP BFP.1 99.8% 99.5% 716A>N
2 CONTROL eGFP eGFP Hahn Lab eGFP eGFP.1 99% 97.9% (many diffs)
3 CONTROL GFP GFP clonetechGfp clonetechGfp.1 99% 97.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
786
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
121agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
181ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
241ggcccaccct cgtgaccacc cTGAGCCACG GCGTCCAGTG CTTCAGCCGC TACCCCGACC
301ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
361CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
421ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
481TGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT CTATATCATG GCCGACAAGC
541AGAAGAACGG CATCAAGGCG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC
601AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG
661ACAGCCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC
721ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT
781ACANGNNGCC NANTNTNTTN TGATTGCCTT TTTAAAAAAN AAAGTGGTGG GTACCCCCCC
841CCCCCCCCCT CTCTTTGGTT TCTATTTAAG AAAATAATAA GGAACACCAG TGGGGTGTTA
901AGTAAAGTCA ATCATCAACT TGAAATAAAA AAAGAAAAGA AAGAAATGGT ATTTTTATTA
961ATTTTGCTCC TCTTATGCTA TGAGGATAAT ACGCTAATAT AATGCAAAAT ATTTCTTTAA
1021ATA

Download FASTA (ORF) (Full)