Construct: ORF ccsbBroad301_99997

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00005.1_s301c1, pLX301-eGFP, BRDN0000462458
Derived from:
BRDN0000464774
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
eGFP (-44)

Vector Information:

Vector Backbone:
pLX_TRC301
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
CMV-ccsbBroad301_99997
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 CONTROL -44 eGFP Hahn Lab eGFP eGFP.1 100% 100% N/A
2 CONTROL -10 GFP clonetechGfp clonetechGfp.1 99.7% 99.5% 93C>T;491T>C
3 CONTROL -40 BFP Hahn Lab BFP BFP.1 99.1% 98.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
786
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
121agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg
181ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
241ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
301acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
361ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
421acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
481tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
541agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
601agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
661acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
721acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
781acaagtcccc aactttcttg tacaaagtgg tttagtaatg agctagcgct aaccggtggc
841gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)