Construct: ORF ccsbBroad301_99995

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00004.2_s301c1, BRDN0000462455
Derived from:
BRDN0000464771
DNA Barcode:
None
Vector:
pLX_TRC301
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
Hahn Lab LacZ

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Note
1 CONTROL -42 LacZ LacZ LacZ.1 99.9% 99.9% 3205_3207delTTG
2 CONTROL -15 lacZ lacZ lacZ.1 91.7% 92.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3273
ORF length:
3204
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gatagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
121cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
181cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct
241ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
301atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
361acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
421gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
481tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
541gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa
601accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
661tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa
721tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg
781ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc
841agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
901gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga
961gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
1021tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
1081tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc
1141tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga
1201acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt
1261gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
1321caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc
1381gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat
1441caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc
1501gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga
1561tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
1621aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga
1681tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt
1741tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg
1801gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct
1861gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac
1921accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
1981acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
2041cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
2101tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
2161aaccgaacgc gaccgcatgg tcagaagccg gccacatcag cgcctggcag cagtggcgtc
2221tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca
2281ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt
2341caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
2401atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
2461accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt
2521tgttgcagtg cacggcagat acacttgctg acgcggtgct gattacgacc gctcacgcgt
2581ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg
2641gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga
2701ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
2761cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
2821tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
2881gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
2941acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca
3001catggctgaa tatcgacggt ttccatatgg ggattggtgg agacgactcc tggagcccgt
3061cagtatcggc ggaattacag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
3121aaaaaactaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt gatatccagc
3181acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
3241ctctcctcgg tctcgattct acgcgtaccg gtttgccaac tttcttgtac aaagtggttt
3301agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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