Construct: shRNA TRCN0000424848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022358.2-1071s21c1
DNA Barcode:
GCCAGGGTCGAATCTGGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK15 (60598)
Gene Description:
potassium two pore domain channel subfamily K member 15
Transcript:
RefSeq NM_022358.2 (NON-CURRENT)
Match location:
Position 1071 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGGTCGAATCTGGAATG
Hairpin Sequence:
5'-CCGG-GCCAGGGTCGAATCTGGAATG-CTCGAG-CATTCCAGATTCGACCCTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGGTCGAATCTGGAATGCTCGAGCATTCCAGATTCGACCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGGTCGAATCTGGAATGCTCGAGCATTCCAGATTCGACCCTGGC-3'

Other clones with same target sequence:

(none)