Construct: shRNA TRCN0000420606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153450.1-616s21c1
DNA Barcode:
TGGAGCTCTGGACTCCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED19 (219541)
Gene Description:
mediator complex subunit 19
Transcript:
RefSeq NM_153450.1 (NON-CURRENT)
Match location:
Position 616 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGCTCTGGACTCCTAAAT
Hairpin Sequence:
5'-CCGG-TGGAGCTCTGGACTCCTAAAT-CTCGAG-ATTTAGGAGTCCAGAGCTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGCTCTGGACTCCTAAATCTCGAGATTTAGGAGTCCAGAGCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGCTCTGGACTCCTAAATCTCGAGATTTAGGAGTCCAGAGCTCCA-3'

Other clones with same target sequence:

(none)