Construct: shRNA TRCN0000249093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028622.2-512s21c1
DNA Barcode:
TGCTGACAAGTGCCATGAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lce1c (73719)
Gene Description:
late cornified envelope 1C
Transcript:
RefSeq NM_028622.2 (CURRENT)
Match location:
Position 512 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGACAAGTGCCATGAAGA
Hairpin Sequence:
5'-CCGG-TGCTGACAAGTGCCATGAAGA-CTCGAG-TCTTCATGGCACTTGTCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGACAAGTGCCATGAAGACTCGAGTCTTCATGGCACTTGTCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGACAAGTGCCATGAAGACTCGAGTCTTCATGGCACTTGTCAGCA-3'

Other clones with same target sequence:

(none)