Construct: shRNA TRCN0000231764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_692s21c1
DNA Barcode:
CTCTCGGCATGGACGAGCTGT
Vector:
pLKO_TRC005

Original Target:

Taxon:
Control
Gene:
GFP (-10)
Gene Description:
clonetechGfp
Transcript:
clonetech clonetechGfp.1 (CURRENT)
Match location:
Position 692 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -10 GFP clonetechGfp clonetechGfp.1 100% CDS 692 0.000 N/A
2 CONTROL -40 BFP BFP BFP.1 100% CDS 692 0.000 N/A
3 CONTROL -44 eGFP eGFP eGFP.1 100% CDS 692 0.000 N/A
4 mouse 15370 Nr4a1 nuclear receptor subfamily ... NM_010444.2 85% CDS 642 N/A N/A
5 mouse 15370 Nr4a1 nuclear receptor subfamily ... XM_006520455.1 85% CDS 687 N/A N/A
6 mouse 15370 Nr4a1 nuclear receptor subfamily ... XM_006520456.1 85% CDS 774 N/A N/A
7 mouse 15370 Nr4a1 nuclear receptor subfamily ... XM_006520457.1 85% CDS 1473 N/A N/A
8 mouse 15370 Nr4a1 nuclear receptor subfamily ... XM_006520458.1 85% CDS 959 N/A N/A
9 mouse 15370 Nr4a1 nuclear receptor subfamily ... XM_006520459.1 85% CDS 1622 N/A N/A
10 mouse 94181 Nans N-acetylneuraminic acid syn... NM_053179.3 89% CDS 363 N/A N/A
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Sequence Information

Target Sequence:
CTCTCGGCATGGACGAGCTGT
Hairpin Sequence:
5'-CCGG-CTCTCGGCATGGACGAGCTGT-CTCGAG-ACAGCTCGTCCATGCCGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCGGCATGGACGAGCTGTCTCGAGACAGCTCGTCCATGCCGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCGGCATGGACGAGCTGTCTCGAGACAGCTCGTCCATGCCGAGAG-3'

Other clones with same target sequence:

  1. TRCN0000072187