Construct: shRNA TRCN0000231759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_229s21c1
DNA Barcode:
GACCACATGAAGCAGCACGAC
Vector:
pLKO_TRC005

Original Target:

Taxon:
Control
Gene:
GFP (-10)
Gene Description:
clonetechGfp
Transcript:
clonetech clonetechGfp.1 (CURRENT)
Match location:
Position 229 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -10 GFP clonetechGfp clonetechGfp.1 100% CDS 229 0.720 N/A
2 CONTROL -40 BFP BFP BFP.1 100% CDS 229 0.720 N/A
3 CONTROL -44 eGFP eGFP eGFP.1 100% CDS 229 0.720 N/A
4 human 102723494 LOC102723494 uncharacterized LOC102723494 XR_425054.1 89% 3UTR 62 N/A N/A
5 human 102723494 LOC102723494 uncharacterized LOC102723494 XR_426887.1 89% 3UTR 62 N/A N/A
6 human 102723494 LOC102723494 uncharacterized LOC102723494 XR_431328.1 89% 3UTR 62 N/A N/A
7 mouse 229584 Pogz pogo transposable element w... NM_001165948.1 89% CDS 1670 N/A N/A
8 mouse 229584 Pogz pogo transposable element w... NM_172683.3 89% CDS 1697 N/A N/A
9 mouse 229584 Pogz pogo transposable element w... XM_006501362.1 89% CDS 1663 N/A N/A
10 mouse 229584 Pogz pogo transposable element w... XM_006501363.1 89% CDS 1636 N/A N/A
11 mouse 229584 Pogz pogo transposable element w... XM_006501364.1 89% CDS 1504 N/A N/A
12 mouse 13801 Enam enamelin NM_017468.3 89% CDS 739 N/A N/A
13 mouse 73942 Fam151b family with sequence simila... NM_001163627.1 89% CDS 431 N/A N/A
14 mouse 73942 Fam151b family with sequence simila... XM_006517777.1 89% CDS 259 N/A N/A
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Sequence Information

Target Sequence:
GACCACATGAAGCAGCACGAC
Hairpin Sequence:
5'-CCGG-GACCACATGAAGCAGCACGAC-CTCGAG-GTCGTGCTGCTTCATGTGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCACATGAAGCAGCACGACCTCGAGGTCGTGCTGCTTCATGTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCACATGAAGCAGCACGACCTCGAGGTCGTGCTGCTTCATGTGGTC-3'

Other clones with same target sequence:

  1. TRCN0000072190