Construct: shRNA TRCN0000231744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_428s21c1
DNA Barcode:
AGTACAACTACAACAGCCACA
Vector:
pLKO_TRC005

Original Target:

Taxon:
Control
Gene:
GFP (-10)
Gene Description:
clonetechGfp
Transcript:
clonetech clonetechGfp.1 (CURRENT)
Match location:
Position 428 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -10 GFP clonetechGfp clonetechGfp.1 100% CDS 428 2.160 N/A
2 CONTROL -40 BFP BFP BFP.1 95% CDS 428 N/A N/A
3 CONTROL -44 eGFP eGFP eGFP.1 100% CDS 428 2.160 N/A
4 human 22828 SCAF8 SR-related CTD-associated f... NM_001286188.1 89% CDS 1191 N/A N/A
5 human 22828 SCAF8 SR-related CTD-associated f... NM_001286189.1 89% CDS 1155 N/A N/A
6 human 22828 SCAF8 SR-related CTD-associated f... NM_001286194.1 89% CDS 1092 N/A N/A
7 human 22828 SCAF8 SR-related CTD-associated f... NM_001286199.1 89% CDS 1144 N/A N/A
8 human 22828 SCAF8 SR-related CTD-associated f... NM_014892.4 89% CDS 1435 N/A N/A
9 mouse 18676 Phf2 PHD finger protein 2 NM_011078.3 89% CDS 2441 N/A N/A
10 mouse 18676 Phf2 PHD finger protein 2 XM_006516880.1 89% CDS 2358 N/A N/A
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Sequence Information

Target Sequence:
AGTACAACTACAACAGCCACA
Hairpin Sequence:
5'-CCGG-AGTACAACTACAACAGCCACA-CTCGAG-TGTGGCTGTTGTAGTTGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACAACTACAACAGCCACACTCGAGTGTGGCTGTTGTAGTTGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACAACTACAACAGCCACACTCGAGTGTGGCTGTTGTAGTTGTACT-3'

Other clones with same target sequence:

  1. TRCN0000072200