Construct: shRNA TRCN0000231742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_158s21c1
DNA Barcode:
ACGCTGAGTACTTCGAAATGT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 158 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 158 5.625 N/A
2 CONTROL -38 luc2 gi 115342883 bases 141-1793... luc2.1 89% CDS 158 N/A N/A
3 mouse 106042 Prickle1 prickle homolog 1 (Drosophila) NM_001033217.4 89% CDS 993 N/A N/A
Download CSV

Sequence Information

Target Sequence:
ACGCTGAGTACTTCGAAATGT
Hairpin Sequence:
5'-CCGG-ACGCTGAGTACTTCGAAATGT-CTCGAG-ACATTTCGAAGTACTCAGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCTGAGTACTTCGAAATGTCTCGAGACATTTCGAAGTACTCAGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCTGAGTACTTCGAAATGTCTCGAGACATTTCGAAGTACTCAGCGT-3'

Other clones with same target sequence:

  1. TRCN0000072256