Construct: shRNA TRCN0000231740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_162s21c1
DNA Barcode:
TGAGTACTTCGAAATGTCCGT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 162 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 162 0.660 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% CDS 162 0.660 N/A
3 human 158314 LINC00475 long intergenic non-protein... NR_027341.1 85% 3UTR 906 N/A N/A
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Sequence Information

Target Sequence:
TGAGTACTTCGAAATGTCCGT
Hairpin Sequence:
5'-CCGG-TGAGTACTTCGAAATGTCCGT-CTCGAG-ACGGACATTTCGAAGTACTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTACTTCGAAATGTCCGTCTCGAGACGGACATTTCGAAGTACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTACTTCGAAATGTCCGTCTCGAGACGGACATTTCGAAGTACTCA-3'

Other clones with same target sequence:

  1. TRCN0000072257