Construct: shRNA TRCN0000231738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_1935s21c1
DNA Barcode:
CCGTCATAGCGATAACGAGTT

Original Target:

Taxon:
Control
Gene:
lacZ (lacZ)
Gene Description:
lacZ
Transcript:
Broad lacZ.1 (CURRENT)
Match location:
Position 1935 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL lacZ lacZ lacZ lacZ.1 100% CDS 1935 4.050 N/A
2 CONTROL LacZ LacZ Hahn Lab LacZ LacZ.1 100% CDS 1920 4.050 N/A
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Sequence Information

Target Sequence:
CCGTCATAGCGATAACGAGTT
Hairpin Sequence:
5'-CCGG-CCGTCATAGCGATAACGAGTT-CTCGAG-AACTCGTTATCGCTATGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCATAGCGATAACGAGTTCTCGAGAACTCGTTATCGCTATGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCATAGCGATAACGAGTTCTCGAGAACTCGTTATCGCTATGACGG-3'

Other clones with same target sequence:

  1. TRCN0000072235