Construct: shRNA TRCN0000231735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_305s21c1
DNA Barcode:
CCAACGTGACCTATCCCATTA
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
NCBI lacZ
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 305 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 305 10.800 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 100% CDS 290 10.800 N/A
3 human 1021 CDK6 cyclin-dependent kinase 6 NM_001145306.1 85% 3UTR 10271 N/A N/A
4 human 1021 CDK6 cyclin-dependent kinase 6 NM_001259.6 85% 3UTR 10166 N/A N/A
5 mouse 628951 Gm6936 predicted gene 6936 NR_045001.1 80% 3UTR 359 N/A N/A
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Sequence Information

Target Sequence:
CCAACGTGACCTATCCCATTA
Hairpin Sequence:
5'-CCGG-CCAACGTGACCTATCCCATTA-CTCGAG-TAATGGGATAGGTCACGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACGTGACCTATCCCATTACTCGAGTAATGGGATAGGTCACGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACGTGACCTATCCCATTACTCGAGTAATGGGATAGGTCACGTTGG-3'

Other clones with same target sequence:

  1. TRCN0000072236