Construct: shRNA TRCN0000231732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_754s21c1
DNA Barcode:
ACACTCGGATATTTGATATGT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 754 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 754 5.625 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% CDS 754 5.625 N/A
3 human 400673 VMAC vimentin-type intermediate ... NM_001017921.3 89% 3UTR 1053 N/A N/A
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Sequence Information

Target Sequence:
ACACTCGGATATTTGATATGT
Hairpin Sequence:
5'-CCGG-ACACTCGGATATTTGATATGT-CTCGAG-ACATATCAAATATCCGAGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTCGGATATTTGATATGTCTCGAGACATATCAAATATCCGAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTCGGATATTTGATATGTCTCGAGACATATCAAATATCCGAGTGT-3'

Other clones with same target sequence:

  1. TRCN0000072253