Construct: shRNA TRCN0000231721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_1193s21c1
DNA Barcode:
TGTCCGGTTATGTAAACAATC
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 1193 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 1193 10.800 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% CDS 1193 10.800 N/A
3 human 414194 CCNYL2 cyclin Y-like 2 XR_110546.1 80% 3UTR 1602 N/A N/A
4 human 100130992 LOC100130992 uncharacterized LOC100130992 NR_038921.1 85% 3UTR 4842 N/A N/A
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Sequence Information

Target Sequence:
TGTCCGGTTATGTAAACAATC
Hairpin Sequence:
5'-CCGG-TGTCCGGTTATGTAAACAATC-CTCGAG-GATTGTTTACATAACCGGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCCGGTTATGTAAACAATCCTCGAGGATTGTTTACATAACCGGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCCGGTTATGTAAACAATCCTCGAGGATTGTTTACATAACCGGACA-3'

Other clones with same target sequence:

  1. TRCN0000072258