Construct: shRNA TRCN0000231721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_1193s21c1
DNA Barcode:
TGTCCGGTTATGTAAACAATC

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 1193 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL LUCIFERASE LUCIFERASE promegaLuc promegaLuc.1 100% CDS 1193 10.800 N/A
2 CONTROL Luciferase Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 1193 10.800 N/A
3 human 105373418 LOC105373418 uncharacterized LOC105373418 XR_921825.1 89% 3UTR 14 N/A N/A
4 human 105373418 LOC105373418 uncharacterized LOC105373418 XR_922778.1 89% 3UTR 14 N/A N/A
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Sequence Information

Target Sequence:
TGTCCGGTTATGTAAACAATC
Hairpin Sequence:
5'-CCGG-TGTCCGGTTATGTAAACAATC-CTCGAG-GATTGTTTACATAACCGGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCCGGTTATGTAAACAATCCTCGAGGATTGTTTACATAACCGGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCCGGTTATGTAAACAATCCTCGAGGATTGTTTACATAACCGGACA-3'

Other clones with same target sequence:

  1. TRCN0000072258