Construct: shRNA TRCN0000231717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_27s21c1
DNA Barcode:
CGTCGTATTACAACGTCGTGA
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
NCBI lacZ
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 27 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 27 2.640 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 95% CDS 12 N/A N/A
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Sequence Information

Target Sequence:
CGTCGTATTACAACGTCGTGA
Hairpin Sequence:
5'-CCGG-CGTCGTATTACAACGTCGTGA-CTCGAG-TCACGACGTTGTAATACGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACG-3'

Other clones with same target sequence:

  1. TRCN0000072232