Construct: shRNA TRCN0000231707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_755s21c1
DNA Barcode:
CACTCGGATATTTGATATGTG

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 755 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL LUCIFERASE LUCIFERASE promegaLuc promegaLuc.1 100% CDS 755 4.950 N/A
2 CONTROL Luciferase Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 755 4.950 N/A
Download CSV

Sequence Information

Target Sequence:
CACTCGGATATTTGATATGTG
Hairpin Sequence:
5'-CCGG-CACTCGGATATTTGATATGTG-CTCGAG-CACATATCAAATATCCGAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCGGATATTTGATATGTGCTCGAGCACATATCAAATATCCGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCGGATATTTGATATGTGCTCGAGCACATATCAAATATCCGAGTG-3'

Other clones with same target sequence:

  1. TRCN0000072261