Construct: shRNA TRCN0000231704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_2265s21c1
DNA Barcode:
GCGTTGGCAATTTAACCGCCA
Vector:
pLKO_TRC005

Original Target:

Taxon:
Control
Gene:
lacZ (-15)
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 2265 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 2265 0.540 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 100% CDS 2250 0.540 N/A
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Sequence Information

Target Sequence:
GCGTTGGCAATTTAACCGCCA
Hairpin Sequence:
5'-CCGG-GCGTTGGCAATTTAACCGCCA-CTCGAG-TGGCGGTTAAATTGCCAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGGCAATTTAACCGCCACTCGAGTGGCGGTTAAATTGCCAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGGCAATTTAACCGCCACTCGAGTGGCGGTTAAATTGCCAACGC-3'

Other clones with same target sequence:

  1. TRCN0000072241