Construct: shRNA TRCN0000231702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_29s21c1
DNA Barcode:
TCGTATTACAACGTCGTGACT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
NCBI lacZ
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 29 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 29 2.640 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 95% CDS 14 N/A N/A
3 human 285796 PACRG-AS1 PACRG antisense RNA 1 NR_028390.1 89% 3UTR 808 N/A N/A
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Sequence Information

Target Sequence:
TCGTATTACAACGTCGTGACT
Hairpin Sequence:
5'-CCGG-TCGTATTACAACGTCGTGACT-CTCGAG-AGTCACGACGTTGTAATACGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGTATTACAACGTCGTGACTCTCGAGAGTCACGACGTTGTAATACGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGTATTACAACGTCGTGACTCTCGAGAGTCACGACGTTGTAATACGA-3'

Other clones with same target sequence:

  1. TRCN0000072240