Construct: TRCN0000231700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_25s21c1
DNA Barcode:
GCCGTCGTATTACAACGTCGT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
NCBI lacZ
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 25 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% 100% CDS 25 2.160 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 90% 89% CDS 10 N/A N/A
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Sequence Information

Target Sequence:
GCCGTCGTATTACAACGTCGT
Hairpin Sequence:
5'-CCGG-GCCGTCGTATTACAACGTCGT-CTCGAG-ACGACGTTGTAATACGACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTCGTATTACAACGTCGTCTCGAGACGACGTTGTAATACGACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTCGTATTACAACGTCGTCTCGAGACGACGTTGTAATACGACGGC-3'

Other clones with same target sequence:

  1. TRCN0000072239