Construct: TRCN0000231695

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_224s21c1
DNA Barcode:
ATCACAGAATCGTCGTATGCA
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 224 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% 100% CDS 224 3.000 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% 100% CDS 224 3.000 N/A
Download CSV

Sequence Information

Target Sequence:
ATCACAGAATCGTCGTATGCA
Hairpin Sequence:
5'-CCGG-ATCACAGAATCGTCGTATGCA-CTCGAG-TGCATACGACGATTCTGTGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCACAGAATCGTCGTATGCACTCGAGTGCATACGACGATTCTGTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCACAGAATCGTCGTATGCACTCGAGTGCATACGACGATTCTGTGAT-3'

Other clones with same target sequence:

  1. TRCN0000072244