Construct: shRNA TRCN0000231689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_576s21c1
DNA Barcode:
CTACAAGACCGACATCAAGCT
Vector:
pLKO_TRC005

Original Target:

Taxon:
Control
Gene:
RFP (-12)
Gene Description:
RFP
Transcript:
tsien lab genbank rfp.1 (CURRENT)
Match location:
Position 576 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -12 RFP RFP rfp.1 100% CDS 576 2.640 N/A
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Sequence Information

Target Sequence:
CTACAAGACCGACATCAAGCT
Hairpin Sequence:
5'-CCGG-CTACAAGACCGACATCAAGCT-CTCGAG-AGCTTGATGTCGGTCTTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAG-3'

Other clones with same target sequence:

  1. TRCN0000072219