Construct: shRNA TRCN0000231689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_576s21c1
DNA Barcode:
CTACAAGACCGACATCAAGCT
Vector:
pLKO_TRC005

Original Target:

Taxon:
CONTROL
Gene:
tsien lab genbank RFP
Gene Description:
RFP
Transcript:
tsien lab genbank rfp.1 (CURRENT)
Match location:
Position 576 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -12 RFP RFP rfp.1 100% CDS 576 2.640 N/A
2 human 4843 NOS2 nitric oxide synthase 2, in... NM_000625.4 85% CDS 2491 N/A N/A
3 human 10313 RTN3 reticulon 3 NM_006054.2 85% CDS 484 N/A N/A
4 human 10313 RTN3 reticulon 3 NM_201428.1 85% CDS 2815 N/A N/A
5 human 10313 RTN3 reticulon 3 NM_201429.1 85% CDS 541 N/A N/A
6 human 10313 RTN3 reticulon 3 NM_201430.1 85% CDS 484 N/A N/A
7 human 283459 GATC glutamyl-tRNA(Gln) amidotra... NR_033684.1 85% 3UTR 3806 N/A N/A
8 human 648987 LOC648987 uncharacterized LOC648987 NR_034085.1 85% 3UTR 2431 N/A N/A
9 mouse 11687 Alox15 arachidonate 15-lipoxygenase NM_009660.3 89% CDS 1475 N/A N/A
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Sequence Information

Target Sequence:
CTACAAGACCGACATCAAGCT
Hairpin Sequence:
5'-CCGG-CTACAAGACCGACATCAAGCT-CTCGAG-AGCTTGATGTCGGTCTTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAG-3'

Other clones with same target sequence:

  1. TRCN0000072219