Clone Details: TRCN0000222819 (shRNA)
Clone Information:
- Clone ID:
- TRCN0000222819
- Vector:
- pLKO.1
Original Target:
- Taxon:
- CONTROL
- Gene:
- evrogen tGFP
- Gene Description:
- turboGfp
- Transcript:
- evrogen turboGfp.1 (CURRENT)
- Match location:
- Position 510 (CDS)
Current transcripts matched by this hairpin:
| Taxon | Gene | Symbol | Description | Transcript | Match %[?] Percent match of hairpin target sequence (21mer) to transcript RNA sequence. |
SDR Match %[?] Percent match of the "Specificity-Defining Region" of the hairpin target sequence to transcript RNA sequence. The SDR is defined as the initial 19 bases of the (21mer) target sequence.
|
Region | Start Pos.[?] Start position of hairpin's match to the targetted transcript sequence (1-based). |
Intrinsic Score[?] Also called "original score", this assesses the target sequence for predicted cloneability and knockdown performance. |
Adjusted Score[?] Also called "specificity score", this is a function of intrinsic score, specificity factor, and a third factor relating to the specificity of the microRNA seed region of the target sequence. |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | -11 | tGFP | turboGfp | turboGfp.1 | 100% | 100% | CDS | 510 | N/A | N/A |
Sequence Information
- Target Sequence:
- CGGCTACTACAGCTCCGTGGT
- Hairpin Sequence:
- 5'-CCGG-CGGCTACTACAGCTCCGTGGT-CTCGAG-ACCACGGAGCTGTAGTAGCCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGCTACTACAGCTCCGTGGTCTCGAGACCACGGAGCTGTAGTAGCCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGCTACTACAGCTCCGTGGTCTCGAGACCACGGAGCTGTAGTAGCCG-3'