Construct: TRCN0000207152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_668s1c1
DNA Barcode:
AGTTCGTGACCGCCGCCGGGA
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
Clontech GFP
Gene Description:
clonetechGfp
Transcript:
clonetech clonetechGfp.1 (CURRENT)
Match location:
Position 668 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -10 GFP clonetechGfp clonetechGfp.1 100% 100% CDS 668 0.000 N/A
2 CONTROL -40 BFP BFP BFP.1 100% 100% CDS 668 0.000 N/A
3 CONTROL -44 eGFP eGFP eGFP.1 100% 100% CDS 668 0.000 N/A
4 human 100652948 LOC100652948 uncharacterized LOC100652948 XR_132783.1 85% 89% 3UTR 368 N/A N/A
5 human 100653287 LOC100653287 uncharacterized LOC100653287 XR_132946.1 85% 89% 3UTR 368 N/A N/A
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Sequence Information

Target Sequence:
AGTTCGTGACCGCCGCCGGGA
Hairpin Sequence:
5'-CCGG-AGTTCGTGACCGCCGCCGGGA-CTCGAG-TCCCGGCGGCGGTCACGAACT-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCGTGACCGCCGCCGGGACTCGAGTCCCGGCGGCGGTCACGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCGTGACCGCCGCCGGGACTCGAGTCCCGGCGGCGGTCACGAACT-3'

Other clones with same target sequence:

(none)