Construct: shRNA TRCN0000207152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_668s1c1
DNA Barcode:
AGTTCGTGACCGCCGCCGGGA

Original Target:

Taxon:
Control
Gene:
GFP (GFP)
Gene Description:
clonetechGfp
Transcript:
Broad clonetechGfp.1 (CURRENT)
Match location:
Position 668 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000207152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTCGTGACCGCCGCCGGGA
Hairpin Sequence:
5'-CCGG-AGTTCGTGACCGCCGCCGGGA-CTCGAG-TCCCGGCGGCGGTCACGAACT-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCGTGACCGCCGCCGGGACTCGAGTCCCGGCGGCGGTCACGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCGTGACCGCCGCCGGGACTCGAGTCCCGGCGGCGGTCACGAACT-3'

Other clones with same target sequence:

(none)