Construct: shRNA TRCN0000157444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003270.2-920s1c1
DNA Barcode:
GCTGGAGAACTGACAACACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSPAN6 (7105)
Gene Description:
tetraspanin 6
Transcript:
RefSeq NM_003270.2 (NON-CURRENT)
Match location:
Position 920 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGAACTGACAACACTA
Hairpin Sequence:
5'-CCGG-GCTGGAGAACTGACAACACTA-CTCGAG-TAGTGTTGTCAGTTCTCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGAACTGACAACACTACTCGAGTAGTGTTGTCAGTTCTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGAACTGACAACACTACTCGAGTAGTGTTGTCAGTTCTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000312475