Construct: shRNA TRCN0000128593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144982.3-5288s1c1
DNA Barcode:
GCGGAAATTTATTGCATCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFC3H1 (196441)
Gene Description:
zinc finger C3H1-type containing
Transcript:
RefSeq NM_144982.3 (NON-CURRENT)
Match location:
Position 5288 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAAATTTATTGCATCCTT
Hairpin Sequence:
5'-CCGG-GCGGAAATTTATTGCATCCTT-CTCGAG-AAGGATGCAATAAATTTCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAAATTTATTGCATCCTTCTCGAGAAGGATGCAATAAATTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAAATTTATTGCATCCTTCTCGAGAAGGATGCAATAAATTTCCGC-3'

Other clones with same target sequence:

(none)