Construct: shRNA TRCN0000074958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002904.4-1261s1c1
DNA Barcode:
CTGGATTCCTTGTGCCTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NELFE (7936)
Gene Description:
negative elongation factor complex member E
Transcript:
RefSeq NM_002904.4 (NON-CURRENT)
Match location:
Position 1261 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000074958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATTCCTTGTGCCTCATA
Hairpin Sequence:
5'-CCGG-CTGGATTCCTTGTGCCTCATA-CTCGAG-TATGAGGCACAAGGAATCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATTCCTTGTGCCTCATACTCGAGTATGAGGCACAAGGAATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATTCCTTGTGCCTCATACTCGAGTATGAGGCACAAGGAATCCAG-3'

Other clones with same target sequence:

  1. TRCN0000307260