Construct: TRCN0000072264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_160s1c1
DNA Barcode:
GCTGAGTACTTCGAAATGTCC
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 160 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% 100% CDS 160 2.640 N/A
2 CONTROL -18 hlucPlus hlucPlus hlucPlus.1 85% 89% CDS 160 N/A N/A
3 CONTROL -43 Luciferase Luciferase Luciferase.1 100% 100% CDS 160 2.640 N/A
4 mouse 15561 Htr3a 5HT3 serotonin receptor NM_001099644.1 81% 85% CDS 444 N/A N/A
5 mouse 15561 Htr3a 5HT3 serotonin receptor NM_013561.2 81% 85% CDS 444 N/A N/A
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Sequence Information

Target Sequence:
GCTGAGTACTTCGAAATGTCC
Hairpin Sequence:
5'-CCGG-GCTGAGTACTTCGAAATGTCC-CTCGAG-GGACATTTCGAAGTACTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTACTTCGAAATGTCCCTCGAGGGACATTTCGAAGTACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTACTTCGAAATGTCCCTCGAGGGACATTTCGAAGTACTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000231741