Construct: shRNA TRCN0000072264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_160s1c1
DNA Barcode:
GCTGAGTACTTCGAAATGTCC
Vector:
pLKO.1

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (-14)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 160 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 160 2.640 N/A
2 CONTROL -18 hlucPlus gi 42718122 bases 2532-4184... hlucPlus.1 89% CDS 160 N/A N/A
3 CONTROL -43 Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 160 2.640 N/A
4 human 101929657 LOC101929657 uncharacterized LOC101929657 NR_110306.1 89% 3UTR 1998 N/A N/A
5 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_942865.1 89% 3UTR 769 N/A N/A
6 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_942866.1 89% 3UTR 844 N/A N/A
7 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_942867.1 89% 3UTR 705 N/A N/A
8 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_954885.1 89% 3UTR 769 N/A N/A
9 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_954886.1 89% 3UTR 844 N/A N/A
10 human 105377941 LOC105377941 uncharacterized LOC105377941 XR_954887.1 89% 3UTR 705 N/A N/A
11 mouse 15561 Htr3a 5-hydroxytryptamine (seroto... NM_001099644.1 85% CDS 444 N/A N/A
12 mouse 15561 Htr3a 5-hydroxytryptamine (seroto... NM_013561.2 85% CDS 444 N/A N/A
13 mouse 15561 Htr3a 5-hydroxytryptamine (seroto... XM_011242398.1 85% CDS 451 N/A N/A
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Sequence Information

Target Sequence:
GCTGAGTACTTCGAAATGTCC
Hairpin Sequence:
5'-CCGG-GCTGAGTACTTCGAAATGTCC-CTCGAG-GGACATTTCGAAGTACTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTACTTCGAAATGTCCCTCGAGGGACATTTCGAAGTACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTACTTCGAAATGTCCCTCGAGGGACATTTCGAAGTACTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000231741