Construct: TRCN0000072261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_755s1c1
DNA Barcode:
CACTCGGATATTTGATATGTG
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 755 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% 100% CDS 755 4.950 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% 100% CDS 755 4.950 N/A
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Sequence Information

Target Sequence:
CACTCGGATATTTGATATGTG
Hairpin Sequence:
5'-CCGG-CACTCGGATATTTGATATGTG-CTCGAG-CACATATCAAATATCCGAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCGGATATTTGATATGTGCTCGAGCACATATCAAATATCCGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCGGATATTTGATATGTGCTCGAGCACATATCAAATATCCGAGTG-3'

Other clones with same target sequence:

  1. TRCN0000231707