Construct: TRCN0000072259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_159s1c1
DNA Barcode:
CGCTGAGTACTTCGAAATGTC
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 159 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% 100% CDS 159 4.950 N/A
2 CONTROL -38 luc2 luc2 luc2.1 80% 89% CDS 159 N/A N/A
3 CONTROL -43 Luciferase Luciferase Luciferase.1 100% 100% CDS 159 4.950 N/A
4 mouse 666351 Gm13232 predicted gene 13232 XR_140683.1 73% 80% 3UTR 170 N/A N/A
5 mouse 666390 Gm13237 predicted gene 13237 XR_140685.1 73% 80% 3UTR 170 N/A N/A
6 mouse 666474 Gm13167 predicted gene 13167 XR_140684.1 73% 80% 3UTR 170 N/A N/A
7 mouse 666514 Gm13160 predicted gene 13160 XR_140687.1 73% 80% 3UTR 114 N/A N/A
8 mouse 100041490 LOC100041490 high mobility group protein... XR_140686.1 73% 80% 3UTR 114 N/A N/A
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Sequence Information

Target Sequence:
CGCTGAGTACTTCGAAATGTC
Hairpin Sequence:
5'-CCGG-CGCTGAGTACTTCGAAATGTC-CTCGAG-GACATTTCGAAGTACTCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGAGTACTTCGAAATGTCCTCGAGGACATTTCGAAGTACTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGAGTACTTCGAAATGTCCTCGAGGACATTTCGAAGTACTCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000231715