Construct: shRNA TRCN0000072255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_601s1c1
DNA Barcode:
TCTACTGGTCTGCCTAAAGGT

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 601 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL LUCIFERASE LUCIFERASE promegaLuc promegaLuc.1 100% CDS 601 3.000 N/A
2 CONTROL Luciferase Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 601 3.000 N/A
3 mouse 381974 Mrgprg MAS-related GPR, member G NM_203492.2 89% CDS 616 N/A N/A
4 mouse 102633522 Gm31330 predicted gene, 31330 XR_871579.1 89% 3UTR 73 N/A N/A
5 mouse 102633522 Gm31330 predicted gene, 31330 XR_883376.1 89% 3UTR 73 N/A N/A
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Sequence Information

Target Sequence:
TCTACTGGTCTGCCTAAAGGT
Hairpin Sequence:
5'-CCGG-TCTACTGGTCTGCCTAAAGGT-CTCGAG-ACCTTTAGGCAGACCAGTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACTGGTCTGCCTAAAGGTCTCGAGACCTTTAGGCAGACCAGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACTGGTCTGCCTAAAGGTCTCGAGACCTTTAGGCAGACCAGTAGA-3'

Other clones with same target sequence:

  1. TRCN0000231734