Construct: shRNA TRCN0000072252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_154s1c1
DNA Barcode:
ACTTACGCTGAGTACTTCGAA

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (-14)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 154 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 154 3.000 N/A
2 CONTROL -38 luc2 gi 115342883 bases 141-1793... luc2.1 89% CDS 154 N/A N/A
3 CONTROL -43 Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 154 3.000 N/A
4 human 105373671 LOC105373671 uncharacterized LOC105373671 XR_923437.1 85% 3UTR 331 N/A N/A
5 human 105373671 LOC105373671 uncharacterized LOC105373671 XR_929373.1 85% 3UTR 331 N/A N/A
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Sequence Information

Target Sequence:
ACTTACGCTGAGTACTTCGAA
Hairpin Sequence:
5'-CCGG-ACTTACGCTGAGTACTTCGAA-CTCGAG-TTCGAAGTACTCAGCGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACGCTGAGTACTTCGAACTCGAGTTCGAAGTACTCAGCGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACGCTGAGTACTTCGAACTCGAGTTCGAAGTACTCAGCGTAAGT-3'

Other clones with same target sequence:

  1. TRCN0000231733