Construct: shRNA TRCN0000072252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_154s1c1
DNA Barcode:
ACTTACGCTGAGTACTTCGAA
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 154 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% CDS 154 3.000 N/A
2 CONTROL -38 luc2 gi 115342883 bases 141-1793... luc2.1 89% CDS 154 N/A N/A
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Sequence Information

Target Sequence:
ACTTACGCTGAGTACTTCGAA
Hairpin Sequence:
5'-CCGG-ACTTACGCTGAGTACTTCGAA-CTCGAG-TTCGAAGTACTCAGCGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACGCTGAGTACTTCGAACTCGAGTTCGAAGTACTCAGCGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACGCTGAGTACTTCGAACTCGAGTTCGAAGTACTCAGCGTAAGT-3'

Other clones with same target sequence:

  1. TRCN0000231733