Construct: TRCN0000072246

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_221s1c1
DNA Barcode:
CAAATCACAGAATCGTCGTAT
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
promega LUCIFERASE
Gene Description:
promegaLuc
Transcript:
promega promegaLuc.1 (CURRENT)
Match location:
Position 221 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -14 LUCIFERASE promegaLuc promegaLuc.1 100% 100% CDS 221 4.950 N/A
2 CONTROL -43 Luciferase Luciferase Luciferase.1 100% 100% CDS 221 4.950 N/A
3 mouse 320343 Lypd6 LY6/PLAUR domain containing 6 NR_033304.2 77% 85% 3UTR 261 N/A N/A
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Sequence Information

Target Sequence:
CAAATCACAGAATCGTCGTAT
Hairpin Sequence:
5'-CCGG-CAAATCACAGAATCGTCGTAT-CTCGAG-ATACGACGATTCTGTGATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCACAGAATCGTCGTATCTCGAGATACGACGATTCTGTGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCACAGAATCGTCGTATCTCGAGATACGACGATTCTGTGATTTG-3'

Other clones with same target sequence:

  1. TRCN0000231699