Construct: shRNA TRCN0000072234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_14s1c1
DNA Barcode:
CGGATTCTCTGGCCGTCGTAT
Vector:
pLKO.1

Original Target:

Taxon:
Control
Gene:
lacZ (-15)
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 14 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 14 1.650 N/A
2 human 255725 OR52B2 olfactory receptor, family ... NM_001004052.1 85% CDS 433 N/A N/A
3 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_383241.1 85% 3UTR 3048 N/A N/A
4 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_383242.1 85% 3UTR 3028 N/A N/A
5 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_383243.1 85% 3UTR 295 N/A N/A
6 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_383244.1 85% 3UTR 275 N/A N/A
7 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_395653.1 85% 3UTR 3073 N/A N/A
8 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_395654.1 85% 3UTR 3053 N/A N/A
9 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_395655.1 85% 3UTR 320 N/A N/A
10 mouse 102632594 LOC102632594 uncharacterized LOC102632594 XR_395656.1 85% 3UTR 300 N/A N/A
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Sequence Information

Target Sequence:
CGGATTCTCTGGCCGTCGTAT
Hairpin Sequence:
5'-CCGG-CGGATTCTCTGGCCGTCGTAT-CTCGAG-ATACGACGGCCAGAGAATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATTCTCTGGCCGTCGTATCTCGAGATACGACGGCCAGAGAATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATTCTCTGGCCGTCGTATCTCGAGATACGACGGCCAGAGAATCCG-3'

Other clones with same target sequence:

(none)