Construct: shRNA TRCN0000072232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_27s1c1
DNA Barcode:
CGTCGTATTACAACGTCGTGA

Original Target:

Taxon:
Control
Gene:
lacZ (-15)
Gene Description:
lacZ
Transcript:
Broad lacZ.1 (CURRENT)
Match location:
Position 27 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% CDS 27 2.640 N/A
2 CONTROL -42 LacZ Hahn Lab LacZ LacZ.1 95% CDS 12 N/A N/A
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Sequence Information

Target Sequence:
CGTCGTATTACAACGTCGTGA
Hairpin Sequence:
5'-CCGG-CGTCGTATTACAACGTCGTGA-CTCGAG-TCACGACGTTGTAATACGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACG-3'

Other clones with same target sequence:

  1. TRCN0000231717