Construct: TRCN0000072224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_1339s1c1
DNA Barcode:
CGCGATCGTAATCACCCGAGT
Vector:
pLKO.1

Original Target:

Taxon:
CONTROL
Gene:
NCBI lacZ
Gene Description:
lacZ
Transcript:
NCBI lacZ.1 (CURRENT)
Match location:
Position 1339 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript Match %[?] SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -15 lacZ lacZ lacZ.1 100% 100% CDS 1339 0.720 N/A
2 CONTROL -42 LacZ LacZ LacZ.1 100% 100% CDS 1324 0.720 N/A
Download CSV

Sequence Information

Target Sequence:
CGCGATCGTAATCACCCGAGT
Hairpin Sequence:
5'-CCGG-CGCGATCGTAATCACCCGAGT-CTCGAG-ACTCGGGTGATTACGATCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGATCGTAATCACCCGAGTCTCGAGACTCGGGTGATTACGATCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGATCGTAATCACCCGAGTCTCGAGACTCGGGTGATTACGATCGCG-3'

Other clones with same target sequence:

  1. TRCN0000231722