- Construct Type:
- Other Identifiers:
- DNA Barcode:
- tsien lab genbank RFP
- Gene Description:
- tsien lab genbank rfp.1 (CURRENT)
- Match location:
- Position 66 (CDS)
Current transcripts matched by this hairpin:
Percent match of hairpin target sequence (21mer) to transcript RNA sequence.
|SDR Match %[?]
Percent match of the "Specificity-Defining Region" of the hairpin target sequence to transcript RNA sequence. The SDR is defined as the initial 19 bases of the (21mer) target sequence.
Start position of hairpin's match to the targetted transcript sequence (1-based).
Also called "original score", this assesses the target sequence for predicted cloneability and knockdown performance.
Also called "specificity score", this is a function of intrinsic score, specificity factor, and a third factor relating to the specificity of the microRNA seed region of the target sequence.
- Target Sequence:
- Hairpin Sequence:
Oligo design for arrayed cloning:
- Forward sequence:
- Reverse sequence: