Construct: shRNA TRCN0000072209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_188s1c1
DNA Barcode:
CTCAGTTCCAGTACGGCTCCA
Vector:
pLKO.1

Original Target:

Taxon:
Control
Gene:
RFP (-12)
Gene Description:
RFP
Transcript:
tsien lab genbank rfp.1 (CURRENT)
Match location:
Position 188 (CDS)

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL -12 RFP RFP rfp.1 100% CDS 188 0.720 N/A
2 human 155184 SLC2A7 solute carrier family 2 (fa... NM_207420.2 86% CDS 99 N/A N/A
Download CSV

Sequence Information

Target Sequence:
CTCAGTTCCAGTACGGCTCCA
Hairpin Sequence:
5'-CCGG-CTCAGTTCCAGTACGGCTCCA-CTCGAG-TGGAGCCGTACTGGAACTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGTTCCAGTACGGCTCCACTCGAGTGGAGCCGTACTGGAACTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGTTCCAGTACGGCTCCACTCGAGTGGAGCCGTACTGGAACTGAG-3'

Other clones with same target sequence:

  1. TRCN0000231683