Construct: shRNA TRCN0000072195

Construct Description:

Construct Type:
shRNA
Other Identifiers:
clonetechGfp_62s1c1
DNA Barcode:
GCGACGTAAACGGCCACAAGT

Original Target:

Taxon:
Control
Gene:
GFP (GFP)
Gene Description:
clonetechGfp
Transcript:
Broad clonetechGfp.1 (CURRENT)
Match location:
Position 62 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072195)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL GFP GFP clonetechGfp clonetechGfp.1 100% CDS 62 1.650 N/A
2 CONTROL BFP BFP Hahn Lab BFP BFP.1 100% CDS 62 1.650 N/A
3 CONTROL eGFP eGFP Hahn Lab eGFP eGFP.1 100% CDS 62 1.650 N/A
Download CSV

Sequence Information

Target Sequence:
GCGACGTAAACGGCCACAAGT
Hairpin Sequence:
5'-CCGG-GCGACGTAAACGGCCACAAGT-CTCGAG-ACTTGTGGCCGTTTACGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACGTAAACGGCCACAAGTCTCGAGACTTGTGGCCGTTTACGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACGTAAACGGCCACAAGTCTCGAGACTTGTGGCCGTTTACGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000231755