Construct: shRNA TRCN0000045809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014754.1-498s1c1
DNA Barcode:
GCTAGATCCAAATCTTCGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTDSS1 (9791)
Gene Description:
phosphatidylserine synthase 1
Transcript:
RefSeq NM_014754.1 (NON-CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000045809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGATCCAAATCTTCGATA
Hairpin Sequence:
5'-CCGG-GCTAGATCCAAATCTTCGATA-CTCGAG-TATCGAAGATTTGGATCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGATCCAAATCTTCGATACTCGAGTATCGAAGATTTGGATCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGATCCAAATCTTCGATACTCGAGTATCGAAGATTTGGATCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000290942