Construct: shRNA TRCN0000221619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014281.3-730s1c2, TRCN0000017248, NM_014281.3-730s1c1
DNA Barcode:
CGATGACATCAAGAGCGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PUF60 (22827)
Gene Description:
poly(U) binding splicing factor 60
Transcript:
RefSeq NM_014281.3 (NON-CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGACATCAAGAGCGTGTT
Hairpin Sequence:
5'-CCGG-CGATGACATCAAGAGCGTGTT-CTCGAG-AACACGCTCTTGATGTCATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGACATCAAGAGCGTGTTCTCGAGAACACGCTCTTGATGTCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGACATCAAGAGCGTGTTCTCGAGAACACGCTCTTGATGTCATCG-3'

Other clones with same target sequence:

  1. TRCN0000322893