Construct: shRNA TRCN0000004606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022462.x-403s1c1
DNA Barcode:
ATGGCTTACCTGTCGGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HIF3A (64344)
Gene Description:
hypoxia inducible factor 3 subunit alpha
Transcript:
RefSeq NM_022462.2 (NON-CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGCTTACCTGTCGGAGAAT
Hairpin Sequence:
5'-CCGG-ATGGCTTACCTGTCGGAGAAT-CTCGAG-ATTCTCCGACAGGTAAGCCAT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGCTTACCTGTCGGAGAATCTCGAGATTCTCCGACAGGTAAGCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGCTTACCTGTCGGAGAATCTCGAGATTCTCCGACAGGTAAGCCAT-3'

Other clones with same target sequence:

(none)