Construct: BRDN0000464777

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00001.1_s302c1, pLX302-BFP
DNA Barcode:
None
Vector:
pLX_TRC302
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
Hahn Lab BFP

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Note
1 CONTROL -40 BFP BFP BFP.1 99.5% 99.5% 718_720delTTG
2 CONTROL -10 GFP clonetechGfp clonetechGfp.1 99.1% 97.9% (many diffs)
3 CONTROL -44 eGFP eGFP eGFP.1 98.7% 97.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
786
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
121agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
181ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
241ggcccaccct cgtgaccacc ctgagccacg gcgtccagtg cttcagccgc taccccgacc
301acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
361ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
421acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
481tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcatg gccgacaagc
541agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
601agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
661acagccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
721acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
781acaagttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
841gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
901acctctggat ta

Download FASTA (ORF) (Full)