GPP Web Portal - Welcome

The Genetic Perturbation Platform, formerly known as the RNA interference (RNAi) Platform, supports functional investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype.

GPP develops technologies for perturbing genes and assists collaborators in experimental planning and execution by helping choose the best model system and experimental readout to assess the effects of genetic perturbation. Technologies include libraries of short hairpin RNAs (shRNAs), CRISPR/Cas9 constructs, and open reading frames (ORFs) to knock down or overexpress genes, in addition to other techniques such as TALEN and CRISPR/Cas9 for genome editing and "tough decoy" constructs to inhibit microRNAs.

Search

Search by Gene
Search by Clone

Design

Design sgRNAs for transcripts (CRISPR)
Generate matching sgRNA designs for a mouse or human ENSEMBL transcript or target sequence
Design Hairpins for a Target Transcript Sequence (RNAi)
Generate matching shRNA designs for a transcript sequence

Experiment

Protocols
Protocols including clone, DNA, and virus production
Vectors
Description of vectors, maps and sequences

Analyze

Software Tools
Software tools and related resources for screen analysis
Library Resources
Clone inventory downloads

Other Resources

shRNA Library Information
More information on the RNAi Consortium shRNA Library
hORFeome V8.1 ORF Library Information
Access to the hORFeome V8.1 ORF Library from 2011 Nature Methods paper
Frequently Asked Questions