Public TRC Portal - Welcome

Search the TRC shRNA and hORFeome 8.1 ORF libraries

Search by Gene
Search by Clone

sgRNA Design (CRISPR)

Design sgRNAs for transcripts
Generate matching sgRNA designs for a mouse or human ENSEMBL transcript or target sequence

shRNA Design (RNAi)

TRC shRNA design process
Description of the candidate-picking process and rules
Design Hairpins for a Target Transcript Sequence
Generate matching shRNA designs for a transcript sequence

Resources

TRC Library Resources
Clone inventory downloads
Protocols
TRC protocols including clone, dna, and virus production
Vectors
Description of TRC vectors, maps and sequences
Pooled Screening File Downloads
File downloads for analysis of pooled screens
Screening Resources
Helpful resources for using the TRC library in screening, including control clones and protocols for lentiviral infection of many cell lines
Software Tools
Software tools and resources for screen analysis
Frequently Asked Questions