GPP Web Portal - Welcome

The Genetic Perturbation Platform, formerly known as the RNA interference (RNAi) Platform, supports functional investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype.

GPP develops technologies for perturbing genes and assists collaborators in experimental planning and execution by helping choose the best model system and experimental readout to assess the effects of genetic perturbation. Technologies include libraries of short hairpin RNAs (shRNAs), CRISPR/Cas9 constructs, and open reading frames (ORFs) to knock down or overexpress genes, in addition to other techniques such as TALEN and CRISPR/Cas9 for genome editing and "tough decoy" constructs to inhibit microRNAs.

Search the TRC shRNA and hORFeome 8.1 ORF libraries

Search by Gene
Search by Clone
shRNA Library Information
More information on the RNAi Consortium shRNA Library
hORFeome V8.1 ORF Library Information
Access to the hORFeome V8.1 ORF Library from 2011 Nature Methods paper

sgRNA Design (CRISPR)

Design sgRNAs for transcripts
Generate matching sgRNA designs for a mouse or human ENSEMBL transcript or target sequence

shRNA Design (RNAi)

TRC shRNA design process
Description of the candidate-picking process and rules
Design Hairpins for a Target Transcript Sequence
Generate matching shRNA designs for a transcript sequence

Resources

TRC Library Resources
Clone inventory downloads
Protocols
TRC protocols including clone, DNA, and virus production
Vectors
Description of TRC vectors, maps and sequences
Pooled Screening File Downloads
File downloads for analysis of pooled screens
Screening Resources
Helpful resources for using the TRC library in screening, including control clones and protocols for lentiviral infection of many cell lines
Software Tools
Software tools and resources for screen analysis
Frequently Asked Questions