Comparison of Cas9 activators in multiple species.

Nat Methods
Authors
Abstract

Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.

Year of Publication
2016
Journal
Nat Methods
Volume
13
Issue
7
Pages
563-7
Date Published
2016 Jul
ISSN
1548-7105
URL
DOI
10.1038/nmeth.3871
PubMed ID
27214048
PubMed Central ID
PMC4927356
Links
Grant list
F32 GM113395 / GM / NIGMS NIH HHS / United States
P50 HG005550 / HG / NHGRI NIH HHS / United States
RM1 HG008525 / HG / NHGRI NIH HHS / United States
T32 CA009216 / CA / NCI NIH HHS / United States