Comparison of Cas9 activators in multiple species.
Nat Methods
Authors | |
Abstract | Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression. |
Year of Publication | 2016
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Journal | Nat Methods
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Volume | 13
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Issue | 7
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Pages | 563-7
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Date Published | 2016 Jul
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ISSN | 1548-7105
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URL | |
DOI | 10.1038/nmeth.3871
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PubMed ID | 27214048
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PubMed Central ID | PMC4927356
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Links | |
Grant list | F32 GM113395 / GM / NIGMS NIH HHS / United States
P50 HG005550 / HG / NHGRI NIH HHS / United States
RM1 HG008525 / HG / NHGRI NIH HHS / United States
T32 CA009216 / CA / NCI NIH HHS / United States
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