Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system".

RNA
Authors
Keywords
Abstract

The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3' decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.

Year of Publication
2016
Journal
RNA
Volume
22
Issue
5
Pages
660-6
Date Published
2016 May
ISSN
1469-9001
URL
DOI
10.1261/rna.055095.115
PubMed ID
26968626
PubMed Central ID
PMC4836641
Links
Grant list
U54HG00306 / HG / NHGRI NIH HHS / United States