Acute Metabolic Influences on the Natriuretic Peptide System in Humans.

J Am Coll Cardiol
Authors
Keywords
Abstract

BACKGROUND: The cardiac natriuretic peptides (NPs), atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP), have central roles in sodium and blood pressure regulation. Extracardiac factors (e.g., obesity and diabetes) influence NP production, potentially altering cardiovascular responses to volume and pressure stress.

OBJECTIVES: This study examined the effects of acute carbohydrate intake on the NP system in humans, and investigated underlying mechanisms.

METHODS: Normotensive subjects (N = 33) were given a high-carbohydrate shake. Venous blood was sampled to measure N-terminal (NT)-proANP and NT-proBNP levels. Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and HepG2 cells were treated with glucose, and expression levels of NPs and micro ribonucleic acid 425 (miR-425), a negative regulator of ANP, were examined. The role of nuclear factor kappa B (NF-κB) in the glucose-mediated effects was investigated using a NF-κB inhibitor and expression plasmids encoding NF-κB subunits.

RESULTS: We observed a 27% reduction in the levels of circulating NT-proANP (p 

CONCLUSIONS: Acute carbohydrate challenge is associated with a reduction in ANP production. The mechanism appears to involve a glucose-induced increase in the expression of miR-425, mediated by NF-κB signaling.

Year of Publication
2016
Journal
J Am Coll Cardiol
Volume
67
Issue
7
Pages
804-12
Date Published
2016 Feb 23
ISSN
1558-3597
URL
DOI
10.1016/j.jacc.2015.11.049
PubMed ID
26892417
PubMed Central ID
PMC4941828
Links
Grant list
R01-DK-082971 / DK / NIDDK NIH HHS / United States
R01-HL-102780 / HL / NHLBI NIH HHS / United States
P30 DK079626 / DK / NIDDK NIH HHS / United States
T32 HL007208 / HL / NHLBI NIH HHS / United States
R01 HL086875 / HL / NHLBI NIH HHS / United States
T32HL007208 / HL / NHLBI NIH HHS / United States
UL1 RR025758 / RR / NCRR NIH HHS / United States
R01 DK082971 / DK / NIDDK NIH HHS / United States
R01-HL-086875 / HL / NHLBI NIH HHS / United States
1 UL1 RR025758 / RR / NCRR NIH HHS / United States
R01 HL102780 / HL / NHLBI NIH HHS / United States