An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites.

J Hematol Oncol

Authors	Mónica Ferreira Christian Bergmann Isabelle Bodensiek Kristina Peukert Jessica Abert Rafael Kramann Paul Kachel Björn Rath Stephan Rütten Ruth Knüchel Benjamin Ebert Horst Fischer Tim Brümmendorf Rebekka Schneider
Keywords	Animals Humans Bone Marrow Cells Cell Differentiation Hematopoietic Stem Cells Cell Proliferation Reproducibility of Results Hematopoiesis Mice, Inbred C57BL Cells, Cultured Osteogenesis Proteoglycans Bone Marrow Microscopy, Electron Collagen Tissue Engineering Drug Combinations Stem Cell Transplantation Stem Cell Niche Mesenchymal Stromal Cells Laminin Calcium Phosphates Tissue Scaffolds
Abstract	BACKGROUND: Bone marrow (BM) niches are often inaccessible for controlled experimentation due to their difficult accessibility, biological complexity, and three-dimensional (3D) geometry. METHODS: Here, we report the development and characterization of a BM model comprising of cellular and structural components with increased potential for hematopoietic recapitulation at ectopic transplantation sites. Cellular components included mesenchymal stromal cells (MSCs) and hematopoietic stem and progenitor cells (HSPCs). Structural components included 3D β-tricalcium phosphate (β-TCP) scaffolds complemented with Matrigel or collagen I/III gels for the recreation of the osteogenic/extracellular character of native BM. RESULTS: In vitro, β-TCP/Matrigel combinations robustly maintained proliferation, osteogenic differentiation, and matrix remodeling capacities of MSCs and maintenance of HSPCs function over time. In vivo, scaffolds promoted strong and robust recruitment of hematopoietic cells to sites of ectopic transplantation, vascularization, and soft tissue formation. CONCLUSIONS: Our tissue-engineered BM system is a powerful tool to explore the regulatory mechanisms of hematopoietic stem and progenitor cells for a better understanding of hematopoiesis in health and disease.
Year of Publication	2016
Journal	J Hematol Oncol
Volume	9
Pages	4
Date Published	2016 Jan 25
ISSN	1756-8722
URL	http://jhoonline.biomedcentral.com/articles/10.1186/s13045-016-0234-9
DOI	10.1186/s13045-016-0234-9
PubMed ID	26810307
PubMed Central ID	PMC4727380
Links	PubMed Google Scholar DOI

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